Metadata for assembly Zm-EP1-REFERENCE-TUM-1.0 Project: EP1 Genome Assembly - Technical University of Munich BioProject: PRJNA360920 The enormous diversity of maize is reflected by a large number of SNPs and substantial structural variation. To remedy the scarcity of sequence resources for the Flint pool, a reference sequence was generated de novo from inbred line EP1. The EP1 reference sequence complements the maize pan-genome with European Flint diversity.

This project is part of the European Maize project (http://www.europeanmaize.net/). This work was funded by the Bavarian State Ministry of the Environment and Consumer Protection (Project BayKlimaFit; http://www.bayklimafit.de/) BioSample: SAMN06216638 Assembly and sequencing: Assembly methods: NRGene de novo assembly. The assembly was done using DeNovoMAGIC 2.0 after which NRGene’s internal maize ancestral genome was used to build pseudo chromosomes from the de novo assembled scaffolds.
Construction of pseudomolecules: Yes Sequencing technologies: Illumina technologies
Sequencing method: Illumina technologies Publication: European Flint reference sequences complement the maize pan-genome Unterseer, Sandra*; Seidel, Michael A.*; Bauer, Eva; Haberer, Georg; Hochholdinger, Frank; Opitz, Nina, Marcon, Caroline; Baruch, Kobi; Manuel Spannagl; Mayer, Klaus F.X.; Schön, Chris-Carolin* These authors contributed equally.